FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir108_3bs_15_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir108_3bs_15_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17950147
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG37950.033.0890115
CACGTCT50150.025.38402614
ACACGTC51250.025.24308813
CACACGT61100.020.60904912
CAGTCAC50600.019.56820527
ATCGGAA65500.019.0681022
GTCTGAA68000.018.82182117
CGCTATG46050.018.05625229
AGTCACC56950.017.38661628
GTCGCTA48500.017.07218627
CGGGACT30450.016.8826391
AGCACAC79550.016.61004810
CGTCTGA78600.016.32730916
TCGGAAG84600.014.8854753
CCAGTCA72150.014.679728526
GAGCACA95350.014.3642639
TCCAGTC89450.014.34733825
GCACACG91150.014.19325711
ATGCGTG35350.014.1523076
GGTAACT59750.013.741816569
Produced by FastQC (version 0.11.2)