FastQCFastQC Report
Tue 6 Mar 2018
HJJH3BGX5_n01_ir108_3bs_15_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJH3BGX5_n01_ir108_3bs_15_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20208982
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG41150.034.54247328
CTCGTAT36900.034.3137342
ACGTCTG47250.034.24295815
TCGTATG36700.033.74833343
GTCACGC44750.031.99579829
TATCTCG46350.028.7330639
TATGCCG42300.027.97583446
ACACGTC59800.027.6336913
CGTATGC43450.027.55246244
CACGTCT63200.026.25624314
CGCCAAT58000.024.62656433
GCCAATA61000.023.58474534
CAGTCAC63400.022.80072627
CACGCCA65400.022.52625831
CACACGT72800.022.5096612
GTCTGAA77950.021.2878317
TCACGCC69800.021.15569530
GTATGCC59900.020.27446645
ATCGGAA83300.019.632642
CCAATAT75350.019.41443635
Produced by FastQC (version 0.11.2)