FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_ox160_1_repeat.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_ox160_1_repeat.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9054186
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120350.132921943507677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT10500.029.2387431
TATGCCG15250.024.4327248
CGGCAAT28600.023.0369681
CGTATGC16150.022.8600546
GGCGGGC20650.022.55205213
CGCGCGT13850.022.4156191
TCGGGCG21700.022.2532127
CGGGCGC21600.021.71988715
ATCGGGC23600.021.3385686
GCGGGCG22300.020.88351814
GGGCGGC23250.019.8798669
CGCGCCT23650.018.523821
TCGTATG20050.018.4131545
GTATGCC20800.018.24504347
ATGCCGT21000.018.23576749
GCGCCTG25200.017.793642
CGGGCAT18900.017.7038571
TAGCCCG26800.017.51215634
GGCAATC36600.016.5864542
TCCGTAT22850.016.46174237
Produced by FastQC (version 0.11.2)