FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_ko60240_2_repeat.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_ko60240_2_repeat.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7297903
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC14050.031.68009844
TATGCCG15000.029.90331346
ATGCCGT18200.024.6432247
GTATGCC19300.023.59814645
TCGTATG19250.023.48116543
GTGTGCG14750.021.7483948
CGGTAGC21150.021.37409233
AGTCACG21450.020.90897228
GGTAGCA22600.020.30896434
GCACACG22300.020.26308811
ACGTCTG23150.019.37234315
CTCGTAT22650.019.34982942
CACACGT22850.019.32473812
CGGGCGC9400.019.0838215
GCCGTCT23250.018.99343949
CGCGTAT7900.018.77581
GTCACGG24350.018.5608529
ACGGTAG24750.018.54335232
GGCGGGC10350.017.9989113
TGCCGTC25450.017.75849248
Produced by FastQC (version 0.11.2)