FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_ko60240_1_repeat.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_ko60240_1_repeat.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10048000
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG22050.025.8092238
GTGCGAG27750.020.38347610
ACCCGTA28000.020.21395332
TGTGCGA31100.018.6315279
CCCGTAA30750.018.51815833
TGCGAGT31400.018.3438611
AAGGCGC31500.018.29773538
CGCGTAT10150.018.0111641
AGGCGCA31800.017.90804939
TAAGGCG31950.017.8212237
GCGAGTC32850.017.43272212
CCCTCGC32650.017.32620466
TGTGTGC35200.016.8531487
TGGCGGG33950.016.56618357
CGGGCGC15250.016.51390615
GCCAGTA25200.016.2883991
CTGACAT45800.016.1168561
GTAAGGC36400.016.02477336
ATGTGTG42000.015.4384546
ACGGGTG39700.015.11742320
Produced by FastQC (version 0.11.2)