FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_ko1240_2_repeat.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_ko1240_2_repeat.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7094209
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161970.2283129803477738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13400.031.41127248
CGTATGC14250.030.02649746
CGCGTAT7650.024.3491171
GTATGCC18700.023.43109747
TCGTATG18400.023.25405145
ATGCCGT19150.022.70001449
CGCGCGT10000.021.7315881
TCGGGCG14150.020.964027
CTCGTAT21450.019.62593744
CGCGCCT18100.019.248361
TGCCGTC23900.018.62130250
ATCGGGC16100.018.4248986
CGGCAAT23250.018.3971121
CGCGGAT11150.018.252741
GGCGGGC15550.017.96963313
GGGCGGC16000.017.4621459
GCGGGCG16100.017.14161714
CGGGCGC16550.017.09241915
CGGGCAT15400.017.0233021
GCCAGTA17850.016.8124871
Produced by FastQC (version 0.11.2)