FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_ko1240_1_repeat.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_ko1240_1_repeat.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8192953
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98920.12073790732108436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC14250.026.39364646
TATGCCG15300.024.8043648
CGTCCGC15950.023.80006233
TCGGGCG18150.023.5636147
TCCGCAC16300.023.28873435
CCGCACA16300.023.08039936
CGCACAT16550.022.72814637
ATCGGGC19600.022.5242736
GGCGGGC18700.021.76804713
CGCGCGT13000.021.7580721
CGCGTAT9250.021.2560731
CGGGCGC19350.021.03707715
GTCCGCA18350.020.31161134
GCGGGCG20100.020.25186314
GTATGCC19100.020.05009347
CGGCAAT27150.019.8200471
TCGTATG19400.019.5651945
GGGCGGC21950.019.170129
ACGTCCG20000.018.98066532
ATGCCGT20200.018.6167849
Produced by FastQC (version 0.11.2)