FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_ko10240_2_repeat.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_ko10240_2_repeat.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10807004
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG18350.022.9320628
TATGCCG18350.021.0579448
CGTATGC18550.020.64787146
CGGGCGC12600.019.98674415
GTACGTA19650.019.84468835
CGTACGT20950.018.94341734
GTGCGAG23800.018.26059710
CGCGTAT11200.017.8630451
GTATGCC22550.017.59491747
ACCCGTA24250.017.3609932
ATGCCGT24550.016.86404249
TACGTAA23450.016.77755436
CCGTACG23700.016.5991933
GGCGGGC15200.016.56826413
TGCGAGT27100.015.90977911
TAAGGCG27650.015.7243137
CCCTCGC27400.015.611937566
GCCAGTA25950.015.55227851
TGTGCGA28750.015.4764469
TCGTATG25250.015.44227545
Produced by FastQC (version 0.11.2)