FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_ko10240_1_repeat.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_ko10240_1_repeat.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9787046
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG19500.021.5794288
CGCGTAT10450.018.8150041
CGTATGC14500.018.56212846
GTGCGAG22750.018.3450410
TATGCCG14100.018.10619448
TGTGCGA25050.016.7983579
ACCCGTA24550.016.72779732
GCCAGTA25950.015.9510631
TGCGAGT26050.015.88877611
TAAGGCG27800.015.64196337
AGGCGCA26900.015.26863239
GTATGCC18700.015.12826147
CCCTCGC26700.015.11627566
AAGGCGC27500.015.061007538
CCCGTAA28500.015.01443633
TGGCGGG28650.014.69252357
TGTGTGC30400.014.636217
TCGTATG18600.014.47032645
GCGAGTC29100.014.46199212
CGGGCGC12350.013.96610915
Produced by FastQC (version 0.11.2)