FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_ko0240_2_repeat.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_ko0240_2_repeat.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7867335
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124310.15800776247611167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13500.029.90539746
CGCGCGT11100.026.1050191
CGTATGC15150.025.96541444
GTATGCC17500.022.87255545
TCGGGCG16650.021.1306217
GGCGGGC16600.020.78120413
ATGCCGT19650.020.54164547
TCGTATG20250.019.93718343
CGGGCAT15400.019.4879531
ATCGGGC18650.019.2344976
CGGGCGC17400.019.03271515
GGGCGGC18850.018.6646869
GCGGGCG17950.018.64184214
CGGCAAT26800.018.535121
CGCGTAT9950.018.0280171
CGCGCCT18250.017.0116861
CGCGGCT17350.016.7011951
CTCGTAT24450.016.51407242
TGCCGTC25350.016.46718248
TCACGTA24000.016.24544330
Produced by FastQC (version 0.11.2)