FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_43-4w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_43-4w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20125084
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG439190.21823014502697233No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG347380.17261045966317456No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC270310.13431496733131648No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTATGCGGAGCCATCCCTCCGC254250.12633487641591956No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA228540.11355977445858113No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT210990.10483931396261502No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGACCGGAATCCTATGATGTTATCC209750.10422316746603394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT27750.035.06982842
CGTATGC29250.033.85666344
TCGTATG30250.033.42217343
TATGCCG32300.030.97976946
ACGTCTG35300.030.78282715
TCACTCG36950.027.64016230
GCCAGTA97250.027.276581
ACATCCG118250.026.69840855
CACGTCT42350.025.90284314
CTATAAG124250.025.46570246
GTCACTC40550.025.4396629
CGCTATG70850.025.22439829
ACACGTC43650.025.05211813
TATAAGC127350.024.95046647
GTCGCTA72350.024.7950227
CGGAGTC131800.024.01170739
AGTCACT42850.023.83238628
CTGGTCG135500.023.34692269
ACATGCG55450.023.3261474
GCGGAGT136600.023.168338
Produced by FastQC (version 0.11.2)