FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_43-4s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_43-4s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19720457
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG229260.11625491234812661No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG220330.11172661972285937No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT214480.1087601570288153No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG202720.10279680638232674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT25250.033.34913642
TCGTATG26450.032.22314543
TATGCCG27850.030.60338846
ATGCGTG63850.026.037746
CATGCGT62350.025.7790345
ACGTCTG35600.025.3882915
TCACTCG34400.025.17503730
ACATGCG64300.025.0508824
GCCAGTA69300.024.7882751
TGCGAGT70000.022.91282711
TTACGGG19200.022.815138
AGTCACT38600.022.4338528
CTGACAT83550.022.3357731
TGCGTGC74750.022.1486477
CGTATGC39050.021.64930344
GTGCGAG75650.021.3838310
GTCACTC40800.021.308929
CAGTCAC40450.021.23584627
CACGTCT42150.021.19759214
GTATGCC41450.020.97820345
Produced by FastQC (version 0.11.2)