FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_43-3w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_43-3w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10888256
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT123460.1133882230542706No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG122500.11250653915558194No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT115340.10593064674452915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT24700.046.10581242
TCGTATG24800.045.63657443
CCCGAAT29300.045.22726435
TATGCCG26500.044.14145746
GTACTCT57700.041.127843
ACGTCTG35150.040.53403515
GTCACTC34350.038.4677729
AGTCACT35150.038.1806528
TGTTACC62850.037.3249113
GTATGCC32200.037.18437645
ACACGTC38950.036.93377313
TTACCTC63500.036.77985815
ATGCCGT30800.036.62824247
CACGTCT39800.035.97179414
CAGTCAC37950.035.45258727
TCTAGTT71200.035.3647167
TAGTTGT67350.035.1332059
CGAATCT34100.035.11590637
TACCTCT67350.034.831216
CTGTTAC64400.034.656731
Produced by FastQC (version 0.11.2)