FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_43-3s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_43-3s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13679216
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG149850.10954575174483684No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT145770.10656312467030274No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG142000.10380711877054942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT16800.033.48365842
TCGTATG17850.032.4769243
TATGCCG19100.030.7124646
GTCACTC22000.026.3446229
ACGTCTG24000.025.15490315
CATGCGT46100.025.0640245
GCCAGTA43400.025.0358681
ATGCGTG46500.024.8484176
ACATGCG46650.024.7685184
AGTCACT23300.024.72659328
TGCGAGT49350.023.2044111
CGCTATG55650.022.5033329
TGCGTGC53000.022.126367
ACACGTC27650.021.9590413
GTCGCTA58150.021.71257227
GTGCGAG53000.021.67113310
CTGACAT59750.021.5911371
CACGTCT29000.021.17488314
ATGCCGT28450.021.10097347
TAAGTCG24650.020.98889
Produced by FastQC (version 0.11.2)