FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_40-4w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_40-4w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13886785
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTTGGGAAGGGAGCTTCGAGGCGGCCGGCCGCGG162650.11712574220742958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT21700.033.7160342
TCGTATG22950.031.57479943
CGCTATG33950.031.09631529
TAAGTCG67250.030.8758479
TATGCCG24200.029.51524746
GTCGCTA35300.029.41628627
TATCTCG24850.028.74860439
CGCGTAT75900.026.7224861
GGTAACT39850.026.23025569
TAATATC29000.024.5157536
GCGTATT85650.024.323842
CTATAAT30300.023.34732233
AGTCGTC90550.023.12169511
CACTATA31350.023.1159831
GCTATGA46250.023.0527430
TTAAGTC89750.022.9432338
AGTCACT32200.022.39303828
GTGGTAA48700.021.25059767
CGGAATA26050.021.18820868
TGGTAAC49650.021.12206368
Produced by FastQC (version 0.11.2)