FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_40-4s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_40-4s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14013215
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140690.10039808851858763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG33200.031.38118229
TAAGTCG39800.029.9856079
GTCGCTA35600.029.45856727
CTCGTAT15150.029.15870942
GGTAACT39400.025.74116969
TCGTATG17850.024.93819843
GCTATGA43250.024.6499630
CGCGTAT49400.024.090171
TATGCCG20150.023.29009246
TGGTAAC47250.022.04850868
CGGGTCT41300.022.0476783
CCCTGTG48250.021.94964663
GCGTATT56600.021.9382152
GTGGTAA48450.021.43090867
AACGCTT52150.020.05364836
GGGTCTG44900.019.742214
GGTCTGA44750.019.191925
GCAACGT56100.018.93806522
ACGCTTG55900.018.82956937
AGTCACT29700.018.70128428
Produced by FastQC (version 0.11.2)