FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_40-3w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_40-3w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12436640
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG34500.031.49948129
GTCGCTA37150.029.25101527
TCGTATG16350.027.01502843
GGTAACT40600.026.50932169
GCTATGA43300.025.89688730
CGGGTCT43000.025.4280873
GGGTCTG42450.025.2697144
CTCGTAT17650.024.24555642
GGTCTGA44850.023.6867735
CGGAATA27650.021.70804268
GTGGTAA50250.021.34939667
TGCCACA52000.021.30156146
CCTATAG23900.021.214888
TGGTAAC51000.021.17096768
GCAACGT55350.020.19162222
CCCTGTG53500.020.1170363
AGCAACG55800.020.09020221
GCGGTAT9150.019.9805451
TTAGAAT52600.019.8698119
GTATCTC53350.019.46899444
Produced by FastQC (version 0.11.2)