FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_40-3s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_40-3s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15846074
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT22550.032.29089442
CGCTATG38650.029.9940629
TCGTATG24450.029.77650543
GTCGCTA41400.028.16588627
TATGCCG26300.027.55059246
CGGGTCT51350.027.0029893
CGTATGC27100.026.8646144
GGTAACT44850.025.9219569
GGTCTGA53000.025.1861235
GGGTCTG56300.024.2612424
TAAGTCG18950.023.2986689
CACCTAT32400.022.89888231
GCTATGA52550.022.3240830
CCCTGTG54900.021.42755763
GTGGTAA54700.021.31663567
TGGTAAC55100.021.1622268
GTTCGTA31000.021.14297513
GGTTAGA64400.020.24816117
GTATGCC36850.020.13094745
GTATCTC65600.020.09217344
Produced by FastQC (version 0.11.2)