FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_4-4w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_4-4w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8457759
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG20050.033.7193349
TCGTATG10300.028.8106243
CGCGTAT24150.027.2823491
CTCGTAT10250.027.27069542
CGCTATG17550.026.73538229
TATGCCG11550.026.58742546
GTCGCTA18300.025.4505427
GGTAACT19200.024.07729169
GCGTATT28250.023.9316332
CGGGTCT26100.022.334613
CGGAATA15050.020.8596268
GCTATGA22550.020.6558330
GGGTCTG28250.020.3906084
AGTCGTC35250.019.37518911
TTAAGTC35250.019.277228
GTGGTAA24450.019.04808267
CCCTGTG24650.018.89297563
GGTCTGA30000.018.7413585
CGTATGC15800.018.1263344
TGGTAAC26550.018.06141568
Produced by FastQC (version 0.11.2)