FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_4-4s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_4-4s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10519517
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGTCTGACACAAGGTTAGAATCCGAGCTCTTCCAGAGTGGTATCTCACTGATGGCTCGGGCCCCCCCGGAA117210.11142146545321426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG25450.028.8745829
CTCGTAT14200.028.18756344
TCGTATG15350.027.64840945
GGTAACT27500.026.72045969
GTCGCTA27700.026.27782227
CGGGTCT38300.026.026493
TATGCCG16100.025.71407748
TAAGTCG16650.025.2735489
GGGTCTG41300.023.3840834
GGTCTGA40100.023.3098435
GCTATGA33400.022.21117830
TGGTAAC34200.021.38487868
CCCTGTG34700.021.07663763
GTGGTAA35700.020.5827967
CGCGTAT21450.020.2622971
TTAGAAT47350.019.887219
GTTAGAA48700.019.26737418
TATCTCG21200.019.2091641
TGGTATC49000.019.08644742
CGTATGC21550.019.0538846
Produced by FastQC (version 0.11.2)