FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_4-3w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_4-3w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9467222
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGTCTGACACAAGGTTAGAATCCGAGCTCTTCCAGAGTGGTATCTCACTGATGGCTCGGGCCCCCCCGGAA121130.12794671974524313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT10900.033.244942
CGCTATG23850.033.12480529
GTCGCTA25450.031.4471427
TCGTATG11650.031.39609143
GGTAACT26650.030.16057869
CGGGTCT39600.026.4797273
TATGCCG14650.026.37965846
GCTATGA31650.025.50796930
GGTCTGA40000.024.921435
CGTATGC15000.024.84424844
GGGTCTG40950.024.6800774
CCCTGTG33850.023.74507763
TGGTAAC34200.023.70418568
TAAGTCG12000.023.5705669
GTGGTAA35350.022.73798867
CGGAATA19500.021.93665168
GCAACGT38100.021.18577422
TTAGAAT47600.020.725219
GTTAGAA48350.020.547718
GTATCTC47950.020.36531844
Produced by FastQC (version 0.11.2)