FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_4-3s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_4-3s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9628820
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG20500.031.63823129
GTCGCTA22150.029.4364227
TCGTATG16850.028.25976845
CGTATGC16700.027.89402646
CGGGTCT29950.026.0277353
TATGCCG18250.025.89771348
ACATTCG15600.025.8692464
CTCGTAT18500.025.73997144
GGTAACT26300.024.92281269
TATCTCG19100.024.3928241
GGGTCTG32250.023.1018374
GCTATGA29100.022.76373530
ATTCGCC19550.022.7596446
CATTCGC19800.021.2528485
CAGTCAC22900.021.09062627
ACGTCTG23600.020.61080715
GTTCGTA16900.019.80014813
GTGGTAA33500.019.56606367
GGTCTGA38300.019.452595
GTATGCC24550.019.25216547
Produced by FastQC (version 0.11.2)