FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_28-4w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_28-4w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14000756
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT356960.25495766085774224No Hit
CAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGCCTACCATGGTGGTGACGGGTGACGGAGAATTAGG320610.22899477713917735No Hit
GTTCTTTTCACCTTTCCCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCAAGGTGGTCC317570.22682346581856005No Hit
ATTTATTGTCACTACCTCCCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCG309860.22131662033107352No Hit
CACGGTTTCACGTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTTTCCCTCACGGTACTACTTCGCTATCG275150.19652510193021006No Hit
GCGGTGGATACCTAGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAA274500.19606084128599913No Hit
GCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGCCTACCATGGTGGTGACGGGTGACGGA273830.19558229569888944No Hit
GCGGAAGCGAGAATGTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAAGGTTTCCT257490.18391149735057163No Hit
CATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGGGGCCTACCATGGTGGTGACGGGTGACGGAG252970.18068310025544335No Hit
CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGGAAT244470.1746119995234543No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG243590.1739834620359072No Hit
GTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCC227850.16274121197455338No Hit
CGCGGGTCTGACACAAGGTTAGAATCCGAGCTCTTCCAGAGTGGTATCTCACTGATGGCTCGGGCCCCCCCGGAA224720.16050561841089153No Hit
CTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCTTGCCTGCTCATGGATTCAGCAGGCA205070.14647066201282272No Hit
CTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCGAAGGTCTAAAGGATCG193140.13794969357368989No Hit
CTTGCGGTGGATACCTAGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAA189350.13524269689436771No Hit
CTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCAAGGTGGTCCTTGCTGATTCACACGGGATTCCACGTGCC188250.13445702503493384No Hit
GTCTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCAC184440.13173574341271285No Hit
CTCAAACTTTAAATAGGTAGGACGGCGCGGCTGCTCCGGTGAGCCGCGCCACGGAATCGGGAGCTCCAAGTGGGC181190.1294144401916582No Hit
CTGGGCACTGTCTCGGAGAGAGACTCGGTGAAATAGACATGTCTGTGAAGATGCGGACTACCTGCACCTGGACAG170170.12154343665442068No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA166980.11926498826206242TruSeq Adapter, Index 19 (97% over 37bp)
GTCACCTTGTGTCCTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGG165260.1180364831727658No Hit
CTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGG164830.11772935690044166No Hit
ATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAAACCTTGG164730.11765793218594767No Hit
GAGCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGCACCCTAGATGGCTAAAGTCCAGTAGCCGA160110.11435811037632539No Hit
CGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAAGTTG157700.11263677475702026No Hit
GCACGGTTTCACGTTCTATTTCACTACCCACTGGGGGTTCTTTTCACCTTTCCCTCACGGTACTACTTCGCTATC156940.11209394692686596No Hit
GCAATACAAGCGTTGTGCTGCTAGGCGAAGCGGTTGAGTGCCGCACCCTAGATGGCTAAAGTCCAGTAGCCGAAA154680.1104797483793018No Hit
TGTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAAAC153300.10949408731928477No Hit
GGGCTTACTACTTATATGCTTTCAGCAGTTATCCTCTCCGCACTTGGCTACCCAGCGTTTACCGTAGGCACGATA152720.10907982397521962No Hit
CGGCGAGCGAAATGGGAGCAGCCTAAACCGTGAAAACGGGGTTGTGGGAGAGCAATACAAGCGTTGTGCTGCTAG151500.10820844245839295No Hit
GAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGAGACTCGGTGAAATAGACATGTCTGTGAAGATGCGGACTA148690.10620140798111187No Hit
GTCTGACACAAGGTTAGAATCCGAGCTCTTCCAGAGTGGTATCTCACTGATGGCTCGGGCCCCCCCGGAAGGGGG144810.1034301290587451No Hit
CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGC144160.10296586841453419No Hit
CCCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCGAAGGTCTAAAGGATCGATAGGCCACGCTTTCACG142950.10210162936915691No Hit
CGAGAATGTCGGCTTGAGTAACGAAAACATTGGTGAGAATCCAATGCCCCGAAAACCCAAGGTTTCCTCCGCAAG142660.10189449769712436No Hit
GGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACG142290.10163022625349659No Hit
CCGGGAACGGATTCACCGCCGTATGGCTGACCGGCGATTACTAGCGATTCCTGCTTCATGCAGGCGAGTTGCAGC141640.10116596560928567No Hit
AAGAAACTTACGAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAGATGCCCAGCTTGAGAATCGGGCGGCCGCG140370.10025887173521202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT21650.063.75269744
TATGCCG22350.063.44428348
GTATGCC22900.061.92095647
TCGTATG22950.061.79377445
GCCGTCT23200.061.2676551
ATGCCGT23500.060.33934849
ACGTGAT24200.058.89195332
CCAATCT23700.058.10549539
TGCCGTC24500.057.87610250
CACGTGA24850.057.490831
GTCACGT25000.057.40633429
AGTCACG25100.057.3150728
CGTGATC25150.056.94202833
CAGTCAC25750.055.99905427
TCCAATC24750.055.64061438
TCACGTG25950.055.44442730
CAATCTC24900.055.30523340
ACGTCTG30400.051.4065815
GAACTCC30200.051.39826621
TGAACTC30400.051.17303520
Produced by FastQC (version 0.11.2)