FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_28-4s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_28-4s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17437397
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT176020.1009439654324553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG25150.028.80701428
CGTATGC26550.027.29230544
CTCGTAT29100.024.66724242
GTCACGA30750.024.12203829
ACGTCTG31450.023.58351715
GTCGCTA66150.022.998527
CGCTATG70200.021.67315729
GCCAGTA44950.021.2573181
CACGTCT35650.021.19216514
ATGCGTG45350.020.7641356
TCGTATG38400.019.94835943
GCTATGA76800.019.94715930
CGGGACT30200.018.9609261
CGGAATA36800.018.27958568
CTATAAG46800.017.91599546
TCTAATG44200.017.79271910
ATCGAAG90100.017.763226
AGCAACG86700.017.62500821
ACCTAGG33400.017.45299510
ACATCCG48250.017.0907555
Produced by FastQC (version 0.11.2)