FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_28-3w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_28-3w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12484476
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT171750.13757085199250652No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG156120.1250513037151099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11450.026.21840946
TCGTATG12000.025.5922443
CTCGTAT12050.024.91592242
GCCAGTA38700.023.4420011
ACATGCG50150.023.177534
CGCTATG64800.022.78751429
ATGCGTG52400.022.4457826
GTCGCTA65650.022.38602427
CATGCGT51650.022.3042585
CTGACAT58000.021.7077481
TTACGGG12850.021.4735438
TGCGAGT54250.021.29938911
GTGCGAG56600.020.71958710
GCTATGA71400.020.6826830
TGCGTGC56700.020.500227
GGTAACT72250.020.19758269
CGGAATA37950.020.08980268
ACGTCTG16900.020.00369315
CCTTACC16650.019.8887231
CCCTGTG76000.019.15575263
Produced by FastQC (version 0.11.2)