FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_28-3s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_28-3s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12649498
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT13900.026.56741142
TCGTATG15050.024.53317343
TATGCCG16550.022.93448846
ATGCGTG42300.021.935026
GCCAGTA40150.021.5653841
CATGCGT42600.021.3757065
ACATGCG43150.021.183184
CGCTATG51550.020.4111629
TTACGGG11400.020.2722158
TGCGAGT44600.020.18545711
GTCGCTA52100.020.12843727
CTATAAG39150.019.03778346
GTGCGAG47900.018.93875910
CTGACAT56300.018.9329871
ACCTAGG30800.018.81437710
CGGGACT24650.018.7524011
ACATCCG40200.018.4532355
TAAGTCG17800.018.4091979
TGCGTGC49800.018.2852427
TACCTAG33350.017.9963599
Produced by FastQC (version 0.11.2)