FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_20-4w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_20-4w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10510069
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTATTTAAGTCGTCTGCAAAGGATTCAGCCCGCCGCCCGTTGGGAAGGGAGCTTCGAGGCGGCCGGCCGCGG126310.12017999120652777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT14000.036.97677242
TCGTATG14450.036.5372343
CGCTATG24050.030.41062729
TAAGTCG50300.029.7613039
TATGCCG18850.028.19171146
GGTAACT25950.027.91668369
GTCGCTA27550.026.92069827
CGGGTCT38150.024.6826693
GCTATGA31850.024.15713930
GGTCTGA38800.023.9135785
GGGTCTG39500.023.8390854
AGTCGTC63650.023.51970511
CGCGTAT64250.023.3010641
GTGGTAA33300.022.16895567
CAGTCAC24700.022.06630527
ACGTCTG26450.022.0422215
GCGTATT68800.021.8590012
CCCTGTG34650.021.50424263
TGGTAAC34500.021.29807368
CGGAATA18250.021.17068768
Produced by FastQC (version 0.11.2)