FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_20-4s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_20-4s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12236019
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT15950.033.32025542
CGCTATG29400.029.92295529
TCGTATG18400.029.81710243
TATGCCG19400.028.81266646
GTCGCTA33100.027.09767727
GGTAACT32650.026.94352169
TAAGTCG23800.026.0870829
TATCTCG22450.023.36705239
GCTATGA41400.021.41848430
CGCGTAT31000.021.1424621
GTGGTAA42600.020.9739667
CCCTGTG41150.020.8748563
GGTCTGA40750.020.6533455
TGGTAAC43000.020.61848368
CGGGTCT42900.020.5026993
TAGCCGG22100.020.4460289
CGTATGC26700.020.28956644
CGGAATA18950.020.02513968
GGGTCTG43750.019.5523974
CCTATAG22150.019.1540818
Produced by FastQC (version 0.11.2)