FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_20-3w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_20-3w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9924705
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGTCTGACACAAGGTTAGAATCCGAGCTCTTCCAGAGTGGTATCTCACTGATGGCTCGGGCCCCCCCGGAA130280.13126838530717033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG25450.030.63635429
GTCGCTA28000.027.84457427
CGGGTCT41450.026.1296753
CTCGTAT7800.026.10362242
GGTAACT29550.026.03379869
GGTCTGA40550.026.029125
GGGTCTG41750.025.528834
GCTATGA34050.023.00148230
CCTATAG22700.021.7291878
CCCTGTG36350.021.54381263
GTGGTAA37450.020.81837367
TCGTATG9850.020.66911943
CGGAATA19250.020.6091268
TAGCCGG15900.020.6089279
TATGCCG10900.020.5773146
TAGTGTG24150.020.28354612
GTTAGAA51750.020.26480718
TTAGAAT51250.020.1916419
TGGTAAC38200.020.13881168
AGAGTGG52650.020.12317538
Produced by FastQC (version 0.11.2)