FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_20-3s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_20-3s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12298004
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGTCTGACACAAGGTTAGAATCCGAGCTCTTCCAGAGTGGTATCTCACTGATGGCTCGGGCCCCCCCGGAA133910.1088875885875464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT14500.031.89438242
TCGTATG16350.029.75866143
CGCTATG29350.029.15252129
GGTAACT32200.026.56890169
GTCGCTA32100.026.54550227
TATGCCG19350.025.5004446
TAAGTCG14450.024.3470639
CGGGTCT44650.023.560943
GGGTCTG43450.023.0207214
GCTATGA38500.022.6741630
CGTATGC21350.022.62733344
GGTCTGA44500.022.2451925
CGGAATA19550.021.35047768
CCCTGTG41950.020.55848763
TGGTAAC42850.020.12603268
GTGGTAA42900.020.0218467
AACGCTT46450.019.39361236
GTTCGTA20900.018.65103313
CGCGTAT20650.018.5414491
ACGTCTG27250.018.48257315
Produced by FastQC (version 0.11.2)