FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_12-4w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_12-4w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8380607
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT94660.11295124565559511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG14100.037.1965643
CTCGTAT13900.035.99841342
TATGCCG15400.032.9355246
CGTATGC15650.032.85033844
ACGTCTG16350.032.07068315
ACACGTC17950.029.78910313
CACGTCT19350.027.45537214
CGCTATG31750.025.9702129
GTCGCTA32600.025.07961827
ACTCAAT20250.024.87939635
CTATAAG25900.023.8463146
ATGCCGT22050.023.62484247
CAGTCAC21800.023.4204627
GTATGCC22100.023.26251845
ACATCCG27800.022.46448755
AGTCACC23750.021.78932228
GCTATGA38550.021.65924630
CGTTAAA10900.021.52792533
GGTAACT38050.021.48816969
TATAAGC29000.021.05599647
Produced by FastQC (version 0.11.2)