FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_12-4s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_12-4s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8281542
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT95710.1155702645715013No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG84940.10256544010765144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT11550.023.60474242
ATGCGTG27150.023.376066
CGTATGC12750.022.46255344
TATGCCG13100.022.38988946
ACATGCG28500.022.3896684
CATGCGT28850.022.2377325
TCGTATG12850.022.01935443
ACGATAT12600.021.91091535
GCCAGTA24050.021.801611
ACGTCTG13300.021.78572315
TATCTCG13050.021.15728239
CGATATC13200.020.91736436
CTTAAAC26200.020.8025151
GTCGCTA36000.020.60261327
CGCTATG36000.020.41256329
ACATCCG25100.020.20743655
CTATAAG25500.020.1627846
CTGACAT36900.019.8184451
TTACGGG8100.019.5885128
TGCGAGT32750.019.48437111
Produced by FastQC (version 0.11.2)