FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_12-3w.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_12-3w.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7865310
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT98800.12561488358373668No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG89960.11437565715782341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGTG26700.024.6748266
ACATGCG26650.024.5915344
GTCGCTA39550.024.42360727
CATGCGT26700.024.416455
CGCTATG39900.024.12504829
GCCAGTA26450.023.0832061
TGCGAGT29250.022.99553311
GTGCGAG30500.022.16604610
TGCGTGC29850.021.9555347
GCTATGA46200.021.1350630
CGGAATA23500.020.99220868
ACGTCTG11700.020.9343615
CCCTGTG45300.020.78986463
GGTAACT45300.020.71423769
CTCGTAT6700.020.08827842
CTGACAT36600.019.980361
ACATCCG26200.019.88528855
CGGGACT17200.019.8543321
GTGGTAA49650.019.2463267
AGCAACG51150.019.15243521
Produced by FastQC (version 0.11.2)