FastQCFastQC Report
Fri 9 Mar 2018
HJJ75BGX5_n01_12-3s.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ75BGX5_n01_12-3s.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7917649
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT95880.1210965527772196No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG87020.10990636235579526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT10650.027.86754242
TATGCCG11450.026.82178746
TCGTATG12650.024.2775843
ATGCGTG24900.023.9640756
ACGTCTG14200.023.56536115
CATGCGT25650.022.5910195
ACATGCG26350.021.990884
CGGGACT17150.021.3197981
CACGTCT15900.020.82882714
GCCAGTA22450.020.742391
CGCTATG38100.020.73538829
GTCGCTA39250.020.2151127
TGCGAGT28500.020.21127511
GTGCGAG28700.020.19061510
CGGAATA21300.020.0821868
CTGACAT32200.020.0321271
TAAGTCG11650.019.8365219
ACATCCG23750.019.61320955
TGCGTGC31850.019.384637
ACACGTC17100.019.16517313
Produced by FastQC (version 0.11.2)