FastQCFastQC Report
Fri 9 Mar 2018
HJJ5VBGX5_n01_dei12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ5VBGX5_n01_dei12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16218799
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG458630.28277679500189873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG16150.022.64184648
AACGATC18400.017.24833538
ACGATAC24050.017.21640260
TCGTATG22500.016.86519445
ATACGAT25350.016.33134558
CATACGA25800.016.17951857
GCATACG27650.015.84534756
TACGATA26300.015.742459
CCCGTAT16450.015.5220871
GACGTAT10850.015.26166917
TGAAACG24950.015.072082535
CGATACT27550.014.90418761
CGTATGC25200.014.6475346
CCCGTTT35650.014.6150971
AAACGAT22050.014.54983937
GAAACGA24900.014.547235536
GTCCGTT22200.013.98858451
CTCGAAT51650.013.7619881
CGCCTAT7350.013.6142881
CTCCGAT42900.013.5125171
Produced by FastQC (version 0.11.2)