FastQCFastQC Report
Fri 9 Mar 2018
HJJ5VBGX5_n01_dei11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ5VBGX5_n01_dei11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45194875
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1389620.3074729159003095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAT118350.014.7233351
CTCGTTT135150.014.5015821
ACGTATA34050.013.98129918
GACGTAT33900.013.94141717
ACGATAC64550.013.73796560
CTCGAAT143300.012.9062991
CCCGTTT88050.012.8144961
ATACGAT70250.012.76864358
TACGATA68500.012.69291559
CCCGATT46200.012.5473031
CTCGTAT77200.012.3360151
CTCCGTT123700.012.3292221
CCCGAAT82600.012.3232441
CCCGTAT39550.012.30143451
CATACGA73300.012.18980457
CGATACT74400.011.96557961
GCATACG74900.011.83702856
CTCGATT59950.011.799071
GACTACG42900.011.50020635
ATCCGAA78900.011.45805225
Produced by FastQC (version 0.11.2)