FastQCFastQC Report
Fri 9 Mar 2018
HJJ5VBGX5_n01_dei10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ5VBGX5_n01_dei10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24000118
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG696010.29000274081985766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG42300.044.2833648
CGTATGC53550.035.30216246
TCGTATG54300.034.49683845
ACCCGTC56200.033.21715532
CGTCCCG60900.030.53537835
CTCGTAT60350.027.60890444
GTATGCC68400.027.38570647
ATGCCGT70450.026.88278849
GTCCCGA74400.024.62221736
CACCCGT85900.022.81680731
TGCCGTC85300.022.20267750
TCACCCG91550.021.55983730
TCCCGAT82150.020.74485837
ACACGTC94650.020.73802213
GCCGTCT90950.020.70948451
ACGTCTG94300.020.55922115
CGTCTGA96250.020.07109816
CCGATCT76150.019.84174739
CCCGATC78300.019.7377838
CCGTCCC100150.019.29363834
Produced by FastQC (version 0.11.2)