FastQCFastQC Report
Fri 9 Mar 2018
HJJ5VBGX5_n01_dei09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ5VBGX5_n01_dei09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95351932
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2984190.31296586628155576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG148950.038.1908248
TCGTATG186750.030.44237945
CGTATGC196550.028.854346
CTCGTAT229150.024.08694344
GTATGCC248850.023.1781447
AATCTCG252000.021.69744941
ATGCCGT267300.021.57822449
CGTATTA38450.017.5857033
TGCCGTC334000.017.41364550
CGTCTGA362550.016.54706416
GCCGTCT353550.016.48004251
ACGTCTG368600.016.4532515
TCTCGTA333600.016.45224843
ACACGTC371300.016.21245813
ATCTCGT347050.015.73510742
CCCGTAT97800.014.5358251
CACGTCT441400.013.91926614
CACACGT458000.013.26397212
CCGTCTT447950.013.07640452
CTCGTTT255450.013.0617661
Produced by FastQC (version 0.11.2)