FastQCFastQC Report
Fri 9 Mar 2018
HJJ5VBGX5_n01_dei08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ5VBGX5_n01_dei08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40668276
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1270640.31244009458379796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTTT112200.013.9620071
CTCCGAT101750.013.8360011
ACGTATA29900.013.49903918
GACGTAT30550.013.32473117
CCCGTTT80700.013.0837651
CCCGTAT36750.013.0509561
CTCGTAT69050.012.492841
CCCGATT39300.012.2919371
GACTACG37050.012.01219235
CTCCGGT110400.011.62676051
CGTTCGA30650.011.59279144
CGTATAG35700.011.40267719
CTCCGTT109550.010.835051
CCCGAAT66700.010.8119811
CTCGAAT112500.010.7042871
ACGATAC47450.010.47152860
CTCCTAT108850.010.4292311
TACGATA46750.010.25842459
CTCGCGT17250.010.2015441
CTCCCGT112600.010.14318751
Produced by FastQC (version 0.11.2)