FastQCFastQC Report
Fri 9 Mar 2018
HJJ5VBGX5_n01_dei07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ5VBGX5_n01_dei07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33110676
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG896940.27089147923165324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATA26100.020.22256718
GACGTAT26450.019.95517
GACTACG32250.016.90236335
CTCCGAT88300.016.3731331
CGTATAG32100.016.1203419
CTCCCGT92800.015.9138131
CTCGTTT98650.015.040071
CTCCGGT90850.014.5083131
ACGTCTA38650.014.36988239
CTCCGTT97050.014.1503111
CCCGTAT30750.014.1385181
CCCGTTT75150.013.8202541
CTACGTC39700.013.72945337
CTCCTAT100000.013.525851
AGACGTA40100.013.42044416
ACGATAC43800.013.31396760
CTCGTAT56850.013.1706671
CGCCTAT17650.012.9026151
TACGATA45500.012.81553559
ACTACGT42900.012.7859936
Produced by FastQC (version 0.11.2)