FastQCFastQC Report
Fri 9 Mar 2018
HJJ5VBGX5_n01_dei06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ5VBGX5_n01_dei06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32139485
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG781050.24301882870867408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37500.032.0130146
TCGTATG53900.022.7204443
CGTATGC56850.021.1167644
GACGTAT33300.019.89071317
ACGTATA34400.019.15438818
CGTATAG36500.018.33612819
CTCGTAT62850.018.16766742
GTATGCC73600.016.45158845
ATGCCGT76500.016.2788247
CCCGTAT33800.015.3123651
AATCTCG77950.015.31218439
GACTACG46300.014.7540335
AGACGTA46050.014.608316
CGTATTA16800.014.579633
CGCCTAT16400.014.30647851
ACTACGT48100.013.77083736
CTCGTTT113100.013.5452831
TGCCGTC94050.013.53459948
CTCCGAT94300.013.0624371
GCCGTCT95600.012.73778749
Produced by FastQC (version 0.11.2)