FastQCFastQC Report
Fri 9 Mar 2018
HJJ5VBGX5_n01_dei05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ5VBGX5_n01_dei05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3850761
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95370.24766533160588258No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG93660.2432246509196494TruSeq Adapter, Index 7 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13600.057.57813346
TCGTATG15500.049.40738743
CGTATGC15950.049.09483344
CTCGTAT16200.045.56898542
ATGCCGT17550.044.61895447
GTATGCC18350.043.04968645
TGCCGTC18700.042.42786848
CGTCTGA20250.040.03256616
GCCGTCT19900.039.6960649
ACGTCTG20750.039.0679315
ACACGTC21000.038.76760513
CACACGT22300.036.66325812
TCTCGTA20800.035.4912341
CACGTCT23600.034.7884914
GATCGGA23350.034.431671
ATCTCGT21600.034.01703640
GCACACG24750.033.73088511
ATCGGAA24050.033.7094422
CCGTCTT25350.031.84218650
GATCATC25100.030.09823636
Produced by FastQC (version 0.11.2)