FastQCFastQC Report
Fri 9 Mar 2018
HJJ5VBGX5_n01_dei04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ5VBGX5_n01_dei04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21469542
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4209681.9607684225401736No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3893821.813648376849399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1826150.061.5220831
TATGCCG36300.045.235746
TCGTATG47100.035.08290543
ACGCCAA50800.032.94415332
TATCTCG48950.032.41761439
CGCCAAT52350.031.77068733
CGTATGC52850.031.2660144
GTATGCC54100.030.60736545
TGGGGGG1711050.029.9304241
GTCACGC56400.029.36924729
TGCCGTC60700.027.73397448
CTCGTAT59150.027.41082242
ATGCCGT61400.026.91213247
AGTCACG64900.025.89476228
GCCGTCT67050.025.05602549
CACGCCA70250.024.55975331
TCACGCC71600.024.19324130
CCAATAT74300.022.84571835
ATATCTC77150.022.17801538
ATCTCGT74200.021.71155440
Produced by FastQC (version 0.11.2)