FastQCFastQC Report
Fri 9 Mar 2018
HJJ5VBGX5_n01_dei03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ5VBGX5_n01_dei03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5237717
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG701911.3401067678914307TruSeq Adapter, Index 5 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG279360.5333621499596103No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG208360.3978069070933004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG99050.066.66106446
GTATGCC103450.063.85914245
TCGTATG102950.063.66665643
CGTATGC105150.063.02355644
CTCGTAT95950.062.8471242
TGCCGTC105100.062.429348
ATGCCGT105750.062.40500347
GCCGTCT105700.061.71591649
AGTGATC94100.061.22550236
GATCTCG95050.060.13943539
ATCTCGT96200.059.38408340
TCTCGTA98600.059.1637441
ACGTCTG114000.058.10067415
CCGTCTT113250.057.51014350
CGTCTGA115100.057.48601516
ACACGTC116400.057.0207213
CACGTCT119000.055.7754314
CTTGAAA119450.054.8837160
CACACGT121200.054.81992712
GTCACAC121750.053.85254729
Produced by FastQC (version 0.11.2)