FastQCFastQC Report
Fri 9 Mar 2018
HJJ5VBGX5_n01_dei02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ5VBGX5_n01_dei02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995127
Sequences flagged as poor quality0
Sequence length75
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG32689432.84947549408266TruSeq Adapter, Index 4 (100% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12281212.341339346636158No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG450614.528165751708073No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG365423.672094114620546No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG120451.2103982707734793TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG72470.7282487561889085TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG41710.4191424813114306TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG36650.368294700073458TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG32480.326390500910939TruSeq Adapter, Index 4 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG30450.3059910946040053TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG29830.29976073405706005TruSeq Adapter, Index 4 (100% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG27280.2741358640655916No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGG27280.2741358640655916No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG23070.2318297061581085No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGG21660.21766066039812004No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG21650.217560170711879TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCACTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG20390.20489847024550636TruSeq Adapter, Index 4 (98% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGG18430.18520249174226006No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG17430.17515352311815477TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACG17310.17394764688326214TruSeq Adapter, Index 4 (100% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGG16970.17053099755106635No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG16360.16440112669036214No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG15950.16028104955447897No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGG13560.13626401454286738No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGG13250.13314883426939478No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAGG150.002354412869.0076932
CTCGGAT401.546141E-1068.9972942
GCGCGTA301.131757E-768.99728441
CCGTCTA301.131757E-768.99728450
GAGCGTA150.002355820768.99728441
GAACCGA150.002355820768.99728441
GCGTAAG150.002355820768.99728443
AAGCTCG6500.067.9357939
GCACACG428550.067.5160711
ACACGTC428950.067.4852813
CACACGT428850.067.4768812
CACGTCT428500.067.4353914
CGTCTGA428450.067.435216
TCTGAAC428250.067.4264218
ACGTCTG428500.067.4192815
TCGGAAG431850.067.3596343
GAACTCC426050.067.3454321
ATCGGAA431900.067.335852
GTCTGAA429000.067.33266417
CTGAACT428500.067.3226719
Produced by FastQC (version 0.11.2)