FastQCFastQC Report
Fri 9 Mar 2018
HJJ5VBGX5_n01_dei01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJ5VBGX5_n01_dei01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24237406
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG630520.2601433503238754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG26550.032.0927946
TATCTCG39000.021.40543439
TCGTATG41700.021.09502643
ACGATAC58900.020.85568460
CGTATGC41850.020.689744
CATACGA61000.020.02031357
ATACGAT62550.019.58004858
GCATACG63200.019.21367856
CGATACT65300.018.81171461
CCCGTAT24650.018.4771921
CGACGCG18300.018.10343428
AATCCGA43750.017.50920524
CTCGTAT49800.017.24825142
CGCGACG18650.017.2070526
AAATCCG43000.017.09185623
CCCGATT25650.017.0842271
ATCCGAA43500.017.0547825
ACGCGCG19250.017.0298730
TACGATA72350.016.88186559
CGAATGT79200.016.7698943
Produced by FastQC (version 0.11.2)