FastQCFastQC Report
Fri 11 Mar 2022
HJHWJAFX3_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHWJAFX3_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7033287
Sequences flagged as poor quality0
Sequence length74
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2428533.4529090025758933No Hit
TCTCTCTCTTATACACATCTGACGCTGCCGACGAAGTGGTTAAGGTGTAGATCTCGGTGGTCGCCGTATCATTA427950.6084637240027316Illumina Single End PCR Primer 1 (96% over 30bp)
ACTGTCTCTTATACACATCTGACGCTGCCGACGAAAGGACGCACGTGTAGATCTCGGTGGTCGCCGTATCATTA270820.3850546693174898Illumina RNA PCR Primer (96% over 33bp)
TCTCTCTCTTATACACATCTGACGCTGCCGACGACGTGTACCAGGTGTAGATCTCGGTGGTCGCCGTATCATTA240280.3416325823189072Illumina RNA PCR Primer (96% over 31bp)
ACTGTCTCTTATACACATCTGACGCTGCCGACGAAGGATGTGCTGTGTAGATCTCGGTGGTCGCCGTATCATTA218330.3104238459201224Illumina Single End PCR Primer 1 (96% over 30bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG212810.3025754529852116No Hit
TCTCTCTCTTATACACATCTGACGCTGCCGACGACCTATTGTTAGTGTAGATCTCGGTGGTCGCCGTATCATTA209050.29722944620346076Illumina Single End PCR Primer 1 (96% over 31bp)
ACTGTCTCTTATACACATCTGACGCTGCCGACGAAGTGGTTAAGGTGTAGATCTCGGTGGTCGCCGTATCATTA194500.2765421061304622Illumina Single End PCR Primer 1 (96% over 30bp)
ACTGTCTCTTATACACATCTGACGCTGCCGACGATCACAACAGTGTGTAGATCTCGGTGGTCGCCGTATCATTA189440.2693477459401273Illumina RNA PCR Primer (96% over 30bp)
TCTCTCTCTTATACACATCTGACGCTGCCGACGATACACGTTGAGTGTAGATCTCGGTGGTCGCCGTATCATTA175950.25016752480028187Illumina Single End PCR Primer 1 (100% over 31bp)
ACTGTCTCTTATACACATCTGACGCTGCCGACGATTCGCCACCGGTGTAGATCTCGGTGGTCGCCGTATCATTA155510.22110572197608316Illumina Single End PCR Primer 1 (96% over 30bp)
ACTGTCTCTTATACACATCTGACGCTGCCGACGACCTATTGTTAGTGTAGATCTCGGTGGTCGCCGTATCATTA132810.18883062784157678Illumina Single End PCR Primer 1 (96% over 31bp)
ACTGTCTCTTATACACATCTGACGCTGCCGACGACGTGTACCAGGTGTAGATCTCGGTGGTCGCCGTATCATTA120590.17145610580088655Illumina RNA PCR Primer (96% over 31bp)
ACTCTCTCTTATACACATCTGACGCTGCCGACGAAGTGGTTAAGGTGTAGATCTCGGTGGTCGCCGTATCATTA113650.16158874221967623Illumina Single End PCR Primer 1 (96% over 30bp)
TCTCTCTCTTATACACATCTGACGCTGCCGACGAAGGATGTGCTGTGTAGATCTCGGTGGTCGCCGTATCATTA109690.15595837337506632Illumina Single End PCR Primer 1 (96% over 30bp)
ACTGTCTCTTATACACATCTGACGCTGCCGACGATACACGTTGAGTGTAGATCTCGGTGGTCGCCGTATCATTA102780.14613366410328485Illumina Single End PCR Primer 1 (100% over 31bp)
TCTCTCTCTTATACACATCTGACGCTGCCGACGATTCGCCACCGGTGTAGATCTCGGTGGTCGCCGTATCATTA91650.13030891530517666Illumina Single End PCR Primer 1 (96% over 30bp)
TCTCTCTCTTATACACATCTGACGCTGCCGACGAAAGGACGCACGTGTAGATCTCGGTGGTCGCCGTATCATTA85120.12102449395282747Illumina RNA PCR Primer (96% over 33bp)
TCTCTCTCTTATACACATCTGACGCTGCCGACGATCACAACAGTGTGTAGATCTCGGTGGTCGCCGTATCATTA81580.11599128544022162Illumina RNA PCR Primer (96% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)