FastQCFastQC Report
Fri 11 Mar 2022
HJHWJAFX3_n02_67192_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHWJAFX3_n02_67192_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10888682
Sequences flagged as poor quality0
Sequence length74
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCTGCCGACGATCACAACAGTGTGTAGATCTCGGTGGTCGCCGTATCATTA10333689.49029460131171Illumina RNA PCR Primer (96% over 30bp)
CTGTCTCTTATACACATCTGACGCTGCCGACGATCACAACAGTGTGTAGATCTCGGTGGTCGCCGTATCATTAA1231421.130917405798057Illumina RNA PCR Primer (96% over 30bp)
TCTGTCTCTTATACACATCTGACGCTGCCGACGATCACAACAGTGTGTAGATCTCGGTGGTCGCCGTATCATTA228670.2100070513584656Illumina RNA PCR Primer (96% over 30bp)
ACTGTCTCTTATACACATCTGACGCTGCCGACGATCACAACAGTGTGTAGATCTCGGTGGTCGCCGTATCATTT223130.20491919958724117Illumina RNA PCR Primer (96% over 30bp)
ACTGTCTCTATACACATCTGACGCTGCCGACGATCACAACAGTGTGTAGATCTCGGTGGTCGCCGTATCATTAA118770.10907656225060113Illumina RNA PCR Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)