FastQCFastQC Report
Fri 11 Mar 2022
HJHWJAFX3_n02_67192_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHWJAFX3_n02_67192_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16009614
Sequences flagged as poor quality0
Sequence length74
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCTGCCGACGATACACGTTGAGTGTAGATCTCGGTGGTCGCCGTATCATTA4125372.57680791054675Illumina Single End PCR Primer 1 (100% over 31bp)
CTGTCTCTTATACACATCTGACGCTGCCGACGATACACGTTGAGTGTAGATCTCGGTGGTCGCCGTATCATTAA986880.6164296028623801Illumina Single End PCR Primer 1 (100% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)