FastQCFastQC Report
Fri 11 Mar 2022
HJHWJAFX3_n02_3694_rep3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJHWJAFX3_n02_3694_rep3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14876892
Sequences flagged as poor quality0
Sequence length74
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCTGCCGACGAAGTGGTTAAGGTGTAGATCTCGGTGGTCGCCGTATCATTA11067727.439537774422238Illumina Single End PCR Primer 1 (96% over 30bp)
CTGTCTCTTATACACATCTGACGCTGCCGACGAAGTGGTTAAGGTGTAGATCTCGGTGGTCGCCGTATCATTAA3517602.364472364254577Illumina Single End PCR Primer 1 (96% over 30bp)
TCTGTCTCTTATACACATCTGACGCTGCCGACGAAGTGGTTAAGGTGTAGATCTCGGTGGTCGCCGTATCATTA384530.2584746867826963Illumina Single End PCR Primer 1 (96% over 30bp)
ACTGTCTCTTATACACATCTGACGCTGCCGACGAAGTGGTTAAGGTGTAGATCTCGGTGGTCGCCGTATCATTT210530.1415147733814294Illumina Single End PCR Primer 1 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)